Pharmacologically active flavonoids from the anticancer, antioxidant and antimicrobial extracts of Cassia angustifolia Vahl
نویسندگان
چکیده
BACKGROUND Cassia angustifolia Vahl. (commonly known as senna makkai or cassia senna), native to Saudi Arabia, Egypt, Yemen and also extensively cultivated in Pakistan, is a medicinal herb used traditionally to cure number of diseases like liver diseases, constipation, typhoid, cholera etc. This study was conducted to evaluate the in-vitro antimicrobial, antioxidant and anticancer assays and phytochemical constituents of aqueous and organic extracts of C. angustifolia leaves. METHODS The antimicrobial activities of C. angustifolia aqueous and organic (methanol, ethanol, acetone, ethyl acetate) extracts were investigated by the disk diffusion method. These extracts were further evaluated for antioxidant potential by the DPPH radical scavenging assay. Anticancer activities of the extracts were determined by the MTT colorimetric assay. The total phenolic and flavonoid contents of C. angustifolia extracts were evaluated by the Folin-Ciocalteu method and aluminum chloride colorimetric assay, respectively. The structures of the bioactive compounds were elucidated by NMR and ESI-MS spectrometry. RESULTS Bioactivity-guided screening of C. angustifolia extracts, led to the isolation and identification of three flavonoids quercimeritrin (1), scutellarein (2), and rutin (3) reported for the first time from this plant, showed significant anticancer activity against MCF-7 (IC50, 4.0 μg/μL), HeLa (IC50, 5.45 μg/μL), Hep2 (IC50, 7.28 μg/μL) and low cytotoxicity against HCEC (IC50, 21.09 μg/μL). Significant antioxidant activity was observed with IC50 2.41 μg/mL against DPPH radical. Moreover, C. angustifolia extracts have the potential to inhibit microbial growth of E. cloacae, P. aeruginosa, S. mercescens and S. typhi. CONCLUSION C. angustifolia extracts revealed the presence of quercimeritrin (1), scutellarein (2), and rutin (3), all known to have useful bioactivities including antimicrobial, antioxidant and anticancer activities.
منابع مشابه
بررسی خواص آنتیاکسیدانی و ضدباکتریایی عصارههای متانولی آویشن(Thymus vulgar) ، سنا (Cassia angustifolia) و شیرینبیان (Glycyrrhiza glabra)
Background and Objective: Thymus vulgar, Cassia angustifolia and Glycyrrhiza glabra are widely used in the East Azerbaijan folk medicine. Effective compounds present in these plants could be candidates for some of their biological activities and therefore for their therapeutic uses. In this study, methanolic extract of these plants was used to detect their antioxidant and antimicrobia...
متن کاملThe effect of bio-fertilizers on growth and quantitative yield of Cassia angustifolia Vahl. in Karaj region, Iran
Background & Aim: Cassia angustifolia Vahl is a valuable medicinal plant that it is important for constipation treatment. According to the importance of organic agriculture in increasing the yield of medicinal plants, the effect of bio-fertilizer on quantities yields of Cassia angustifolia Vahl or Senna in Karaj region (Iran) was examined. Experimental: This research was carr...
متن کاملThe effect of bio-fertilizers on growth and quantitative yield of Cassia angustifolia Vahl. in Karaj region, Iran
Background & Aim: Cassia angustifolia Vahl is a valuable medicinal plant that it is important for constipation treatment. According to the importance of organic agriculture in increasing the yield of medicinal plants, the effect of bio-fertilizer on quantities yields of Cassia angustifolia Vahl or Senna in Karaj region (Iran) was examined. Experimental: This research was carr...
متن کاملEffect of different treatments of organic fertilizers on growth characteristics and yield of Cassia angustifolia Vahl.
Background & aim: In sustainable agriculture, organic fertilizers are considered as important alternatives for chemical ones and are of great importance in organic producing of medicinal plants. This study was conducted to study the effects of organic fertilizers on Cassia angustifolia Vahl in dry and subtropical climate. Experimental: This study was carried out in Jiroft region, Ker...
متن کاملEffect of different treatments of organic fertilizers on growth characteristics and yield of Cassia angustifolia Vahl.
Background & aim: In sustainable agriculture, organic fertilizers are considered as important alternatives for chemical ones and are of great importance in organic producing of medicinal plants. This study was conducted to study the effects of organic fertilizers on Cassia angustifolia Vahl in dry and subtropical climate. Experimental: This study was carried out in Jiroft region, Ker...
متن کامل